Application of a contact dehydrating sheet to the

Some enzymes were immobilized in an inner layer of a filter paper using the osmotic action of a contact dehydrating sheet (CDS). CDS was examined to determine if the efficiency and rapidity of an enzyme reaction could be improved, thereby making better of the preparation of an immobilized-enzyme membrane. The activity of the membrane, in which an enzyme exists in its layer, was compared with that of a conventional enzyme adsorption membrane. pH was examinedmodulated for the enzyme to obtain a higher migration rate into the membrane. The membrane in which layer an enzyme exists and the enzyme adsorption membrane were placed on the oxygen electrode. The glucose sensor, the D-amino acid sensor and the sarcosine sensor were constructed. The use of CDS successfully increased enzyme migration into the membrane and enhanced the signal intensity per unit of enzyme. From this point of view, there is a very strong possibility that biosensors utilizing an immobilized-enzyme membrane prepared with CDS can be developed. This is the first report on the application of CDS to bioengineering fields e x c e p t f o r f o o d w r a p p i n g .